海洋产油酵母菌筛选及油脂提取条件优化

摘要

An oleaginous yeast was screened from sea mud and algae attached rock by Sudan Black B staining method and it was identified as Meyerozyma guilliermondii by physicochemical and biochemical characteristics combined with molecular biological methods.The oil was extracted by acid-heating method.Based on single factor experiment,the extraction conditions were optimized by orthogonal experiment.The optimal extraction conditions were obtained as follows:0.5 g of dried cells,4 mol/L HCl 10 mL,acid-heating time 10 min,organic solvent(volume ratio of chloroform to methanol 2∶1) 25 mL,0.15％ NaCl 10 mL.Under these conditions,the oil yield was 40.12％.%采用苏丹黑B染色法,从海洋淤泥和岩石附着藻类中筛选得到一株高产油脂的海洋酵母菌,利用生理生化特征结合分子生物学方法进行目的菌株的鉴定,结果证明其为季也蒙毕赤酵母(Meyerozyma guilliermondii).利用酸热法进行油脂提取,在单因素实验的基础上通过正交实验对提取条件进行优化.得到最优条件为:每0.5g干菌体需4 mol/L盐酸10 mL,酸热时间10 min,有机溶剂氯仿-甲醇(体积比2∶1)25 mL,0.15％NaCl溶液10 mL.在最优提取条件下,油脂得率为40.12％.