褪黑素对雨生红球藻虾青素积累和脂肪酸合成的影响

摘要

The effects of melatonin (MLT)on the algae cell growth,astaxanthin accumulation,fatty acids,and fatty acid synthesis-related enzyme activities of H.pluvialis LUGU were studied.The results showed that the astaxanthin content in algae cells achieved 26.62 mg/g under the induction conditions of MLT concentration 10 μmol/L and cultivation time 13 d,which was 2.16 times as high as that of the control (12.3 mg/g).The content of fatty acids (15.32％) was the highest when cultivating for 5 d,which was 1.24 times as high as that of the control,and the astaxanthin ester content increased.Moreover,the acetyl-CoA carboxylase (ACCase) activity significantly increased,but the phosphoenolpyruvate carboxylase (PEPCase) activity reduced by MLT induction.The study revealed that the appropriate concentration of MLT not only could improve the astaxanthin accumulation in H.pluvialis LUGU,but also could boost the fatty acid content and change the fatty acid composition.%以雨生红球藻H.pluvialis LUGU为对象,研究了不同浓度的褪黑素(MLT)对藻细胞生长、虾青素积累、脂肪酸及脂肪酸合成相关酶活性的影响.结果显示:10 μmol/L MLT诱导条件下培养13 d藻细胞中虾青素含量可达26.62 mg/g,是对照组(12.3 mg/g)的2.16倍;培养5d脂肪酸合成量(15.32％)最大,是对照组的1.24倍,同时提高了虾青素酯含量;MLT诱导条件下,脂肪酸合成关键酶乙酰辅酶A羧化酶(ACCase)活性升高,而磷酸烯醇式丙酮酸羧化酶(PEPCase)活性降低.研究表明,外源添加适当浓度的MLT不仅可以促进雨生红球藻中虾青素的积累,而且提高了脂肪酸的合成量,改变了脂肪酸组成.