复合膳食纤维对急性重症胰腺炎大鼠肠黏膜上皮紧密连接蛋白和肌球蛋白轻链激酶的影响

摘要

Objective To investigate the effect of enteral nutrition (EN) containing dietary fiber complex (DFC) in the intestinal mucosal barrier in mice with severe acute pancreatitis (SAP). Methods SAP mouse model was established with 5% sodium taurocholate which was injected into pancreaticocholang retrogradely. Sixty Wistar mice were randomly assigned into three groups: SAP + enteral nutritional emulsion (fresubin) group (group A), SAP + fresubin and DFC (20 g/L) group [soluble dietary fiber (SDF) : insoluble dietary fiber IDF=2:1, group B], SAP + fresubin and DFC (20 g/L) group (SDF : IDF=1:2, group C). The mice were executed on 48 h after surgery. Pathological intestinal mucosal changes, tight junction protein oe-cludin, ZO-1 and myosin light chain kinase (MLCK) were evaluated in all groups. Results Intestinal mucosal injury in group B and C group was lighter than that in group A (P < 0.05). Intestinal mucosal injury in group C was lighter than that in group B (P < 0.05). Tight junction protein occludin, ZO-1 and myosin light chain kinase (MLCK) in group B and C expressed higher when compared with group C (P < 0.05), furthermore, occluding and ZO-1 in group C expressed higher than those in group B (P < 0.05). MLCK in group C expressed higher than that in group B, but the difference was not statistically significant (P > 0.05). Conclusion Enteral nutrition (EN) containing dietary fiber complex (DFC) plays an important role in the intestinal mucosal barrier in mice with severe acute pancreatitis (SAP), and appropriately increasing insoluble dietary fiber (IDF) is more conducive to the intestinal mucosal barrier protection and ameliorates the damaged intestinal mucosal barrier through regulating tight junction protein occludin, ZO-1 and myosin light chain kinase (MLCK) distribution and expression.%目的 探讨含复合膳食纤维(DFC)肠内营养(EN)对重症急性胰腺炎(SAP)大鼠肠黏膜屏障功能的影响.方法 采用5％的牛黄胆酸钠(0.1 mL/100 mg)逆行注射入胰胆管法制备大鼠重症急性胰腺炎(SAP)模型.60只随机分为三组:SAP+肠内营养乳剂(瑞素)组(A组),SAP+瑞素加复合膳食纤维(20g/L)1组[可溶性膳食纤维(SDF)∶不可溶性膳食纤维(IDF)=2∶1,B组],SAP+瑞素加膳食纤维(20 g/L)2组(SDF∶IDF=1∶2,C组),于术后48 h处死动物,比较各组肠黏膜病理改变,紧密连接蛋白occludin、ZO-1以及肌球蛋白轻链激酶(MLCK)的变化.结果 B组与C组大鼠肠黏膜损伤相对于A组较轻(P＜0.05),C组大鼠肠黏膜损伤相对于B组较轻(P＜0.05).B组与C组大鼠肠黏膜紧密连接蛋白occludin、ZO-1以及肌球蛋白轻链激酶(MLCK)的分布表达相对于A组增高(P＜0.05),且C组occludin及ZO-1的分布和表达高于B组(P＜0.05).而C组MLCK的分布和表达高于B组,但差异无统计学意义(P＞0.05).结论 添加DFC的肠内营养在SAP大鼠肠黏膜屏障功能中起重要的保护作用,且适当加大不可溶性膳食纤维(IDF)的比例更有助于肠道黏膜屏障的保护,改善受损的肠屏障功能.这一作用可能是通过调控紧密连接蛋白occludin、ZO-1以及肌球蛋白轻链激酶(MLCK)的分布和表达来实现的.