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降胆固醇乳酸菌的筛选及其鉴定

             

摘要

tuditudy on 19 Lactobacillus were studied in the experiments. The quantity of the Cholesterol-degrading in MRS-CHOL medium was used to analyze their cholesterol-reducing ability. The results showed that all strains could remove cholesterol from media, and the percentage of Cholesterol-degrading rate ranged from 3.84% to 42.28%. Among them, there were 2 strains exhibited the percentage of Cholesterol-degrading above 40%. Meanwhile, these 2 strains had high removal effectiveness. As a result, these 2 strains were screened for their excellent cholesterol-reducing ability.WE43-1-2 and WE57-1, according to their morphological, physiological and biochetnical properties, including chemical characteristics, were identified to Lactobacillus agilis and Lactobacillus casei subsp casei.%对19株乳杆菌进行体外实验,研究它们在MRS-CHOL培养基中对胆固醇的降解量,进而分析各菌株降胆固醇的能力.结果表明,所有试验菌株均有降解培养物中胆固醇的能力,去除率在3.84%~42.28%之间.其中有2株试验菌对培养物中胆固醇的降解率大于40%,这2株菌对胆固醇的脱除效力也较高.这2株菌WE43-1-2和WE57-1经形态学、生理生化反应以及糖类发酵试验被鉴定为敏捷乳杆菌(Lactobacillus agilis)和干酪乳杆菌干酪亚种(Lactobacillus casei subsp casei).

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