健康奶牛乳中乳酸菌的分离与鉴定

摘要

乳房炎是奶牛养殖业中的顽疾，给奶牛业造成了巨大的经济损失。传统的抗生素疗法存在诸多问题，乳酸菌作为抗生素的替代物在许多治疗乳房炎的案例中取得成功。因此，对于有针对性治疗乳房炎的乳酸菌菌株的筛选显得尤为重要。本试验从20头健康泌乳期荷斯坦牛的乳样中分离细菌，采用16SrRNA基因序列对分离获得的乳酸菌进行鉴定，最终获得黏膜乳杆菌、粪肠球菌、乳酸乳球菌、双歧杆菌等22株乳酸菌。该试验中获得的乳酸菌为今后乳房炎的治疗提供了优良的乳酸菌菌种资源。%Bovine mastitis is the recurring illness of dairy farming, and it bring about huge economic losses to dairy industry. However the traditional antibiotic therapy appears many negative problems, so the lactic acid bacteria(LAB) applied to many cases of the treatment of mastitis have achieved success as the substitutes of antibiotic. Therefore, the screening of LAB which used to treatment of mastitis is particularly important. In this study, milk samples were collected from 20 head of lactation period healthy Holstein cows to isolate and screen bacteria, and use 16S rRNA gene sequences analysis to identify the isolates. As result, we isolate 22 strains of LAB that they were respectively identiifed as Lactobacillus mucosae, Enterococcus faecium, Lactococcus Lactis, Biifdo bacteriumetal. The LAB obtained from the experiment not only enriched the lactic acid bacteria species resources but also provides excellent strains of LAB resources for the future treatment of mastitis test.