响应面法优化光合细菌Rhodospirillum Rubrum S1培养条件

摘要

In the paper,the single factor method is used to study the culture conditions of photosynthetic bacteria (PSB) RhodospirillumRubrum S1,including temperature,light intensity and inoculation quantity.The optimal culture conditions are as follows:temperature of 30 ℃,light intensity of 3000 lx and inoculation quantity of 15％.On the basis of the above conditions,Response Surface Methodology (RSM) is adopted for further optimization of other factors that affect fermentation conditions of PSB Rhodospirillum Rubrum S1.The results show that the optimal temperature is 32.54 ℃,light intensity is 2995.85 lx and inoculation quantity is 15.80％.Besides,the output of carotenoid is 16.72 mg/L,which differs from the theoretical value 16.86 mg/L by 0.83％.As a consequence,RSM to optimize the culture conditions of PSB S1 is reasonable and feasible.%文章首先用单因素法分别对光合细菌Rhodospirillum Rubrum S1的培养条件——温度、光照强度、接种量进行了考察,确定了最佳培养条件:温度30℃,光照强度3000 lx,接种量15％.在此基础上,采用中心组合试验设计结合响应面分析法(Response Surface Methodology,RSM)对影响光合细菌Rhodospirillum Rubrum S1的发酵条件进行进一步优化.结果表明,主要影响因素的最佳条件:培养温度32.54℃,光照强度2995.85 lx,接种量15.80％,光合细菌Rhodospirillum Rubrum S1的类胡萝卜素产量为16.72mg/L,与理论值16.86 mg/L相差0.83％.因此,利用响应面分析法对光合细菌Rhodospirillum Rubrum S1的培养条件进行优化合理可行.