大孔树脂纯化芫荽总黄酮及芫荽总黄酮抗氧化和抗菌活性研究

摘要

Obj ective:To investigate the purification technology of total flavonoids with macroporous resin and to study the antibacterial and antioxidant activity of total flavonoids from Coriandrum sativum L..Method:Screen the optimal macroporous resin and optimize the purification technology of total flavonoids from Coriandrumsativum L.,then use the DPPH method to test the antioxidant activity and agar-diffusion method to test the antibacterial activity of total flavonoids from Coriandrum sativum L..Results:AB-8 resin has excellent effect on the adsorption and desorption of total flavonoids from Coriandrumsativum L..The sample concentration is 1 .5 mg/mL and the rate is 2 mL/min,the eluent is 70% ethanol of 4 BV and rate of 2 mL/min,which are the optimal purification process conditions.The total flavonoids from Coriandrumsativum L.also has excellent antioxidant activity (EC50 = 1 .5 mg/mL ),and the antibacterial activity against Bacillussubtilis, Staphylococcusaureus,Escherichiacoli,Pesudomonasaeruginosa,Aspergillusniger, but not against Saccharomycescerevisiae.%目的：研究芫荽总黄酮的大孔树脂纯化工艺及总黄酮抗氧化、抗菌活性。方法：通过筛选优化大孔树脂型号，优化大孔树脂提取芫荽总黄酮条件，利用DPP H 芫荽总黄酮抗氧化活性，琼脂扩散法测试芫荽总黄酮抗菌活性。结果：AB-8树脂对芫荽总黄酮有较好的吸附和解吸效果，最优工艺条件为：芫荽总黄酮上样液的质量浓度为1．5 mg／mL，上样速率为2 mL／min，洗脱剂为70％乙醇，洗脱剂用量为4 BV，洗脱速率为2 mL／min，芫荽黄酮提取物清除DPPH ·自由基的EC50为1．5 mg／mL。对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌、绿脓杆菌、黑曲霉均有抗菌活性，但是对酿酒酵母无效。结论：AB-8型大孔树脂富集芫荽总黄酮效果最佳。