ELISA法测定热加工食品中的丙烯酰胺

摘要

该研究建立了热加工食品中丙烯酰胺的间接竞争ELISA检测方法.首先用戊二醛法合成丙烯酰胺-BSA免疫抗原,注入兔体内以产生抗丙烯酰胺多克隆抗体.结果显示,在包被抗原为1.5μg/mL,抗体稀释度为1∶16000倍的优化条件下,间接ELISA法检测范围为50μg/L～1280μg/L,IC50为检测限为350μg/L,最低检测限为50μg/L.加标回收率为92.6%～95.5%.该检测方法准确快速,特异性强,适用于热加工食品中丙烯酰胺的快速检测.%Enzyme linked immunosorbent assay(ELISA)was developed to detect acrylamide residue in heated food.Acrylamide-BSA immuno-antigen was prepared by glutaraldehyde method, and was injected into rabbits body to prepare anti-acrylamide-BSA polyclonal antibody.The results showed that the determination limitation was 50μg/L when the concentration of coating antigen was 1.5μg/mL, and the dilution of antibody was 1: 16000.The detection range of the inhibition curve was 50μg/L ～ 1280μg/L.The recovery rate of acrylamide in the samples ranged from 92.6％ to 95.5％.The detection method was very rapid, accurate and had a good specificity.It is suitable for rapid detection of acrylamide in the production of heated food.