高产γ-氨基丁酸乳酸菌的筛选及鉴定

摘要

In this study, using MRS selective medium, six lactic acid bacteria strains producing γ-aminobutyric acid (GABA) were isolated from sauerkraut, pickles, etc.The strain F13, F14 and F15 showed high GABA-producing ability after rescreening.Screening results revealed that the strain F13, F14 and F15 produced 5.01g/L, 5.72g/L and 4.98g/L GABA in the fermentation medium containing 10g/L sodium glutamate under 33℃ and for 60h, respectively.According to the morphological characteristics and physiological and biochemical characteristics, the three strains were preliminarily identified as Lactobacillus brevis, Lactobacillus acidophilus, lactobacillus plantarum, respectively.%利用MRS选择性培养基,从酸菜、泡菜等中分离出6株产γ-氨基丁酸的乳酸菌,经过复筛得到γ-氨基丁酸高产菌株F13、F14和F15.这3株菌在含有10g/L谷氨酸钠的发酵培养基中33℃培养60h,发酵液中γ-氨基丁酸含量分别达到5.72g/L、5.01g/L和4.98g/L.根据乳酸菌的形态特征和生理生化特征,初步鉴定这3株菌分别为短乳杆菌、嗜酸乳杆菌、植物乳杆菌.