Aspergilus niger CICC41254 was treated by ultraviolet radiation for the development of high pectinase-producing strain. Transparent circle method was used for preliminary screening, by which 26 mutant strains were selected with higher ratio of transparent circle diameter to colony diameter on pectin plate media. These mutant strains were re-screened by solid state fermentation in flasks, and one strain named CICC41254-D8 was selected with high pectinase-producing ability. CICC41254-D8 was incubated on slant through 5 generations. It was showed that the genetic expression of pectinase was stable. The pectinase activity could reach 1156.8U/gdry bioaqsis which was 54.24% higher than that of the original strain (750.0U/g).%以黑曲霉(Aspergilus niger) CICC41254为出发菌株,进行紫外线诱变.初筛使用透明圈法,从果胶平板上的突变菌株中,挑取了26株透明圈与菌落直径比值显著大于出发菌株的突变株.将这些突变株进行三角瓶固体发酵复筛,最后筛选出1株高产果胶酶的突变株CICC41254-D8.突变株CICC41254-D8经斜面传代培养了5代,产酶遗传特性稳定,其果胶酶活力最高可达1156.8U/g干基,比出发菌株酶活力(750.0U/g)提高了54.24％.
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