Characterization of iturin high-yielding UV mutant Bacillus subtilis and the improvement of iturin producing Bacillus subtilis by site-directed mutagenesis

摘要

The present invention relates to a method for improving the production of this tyrannin by the property and site-directed mutagenesis of this iturin high-production UV mutant Bacillus subtilis , As a result of first and second ultraviolet treatment of the Bacillus subtilis strain, the productivity of this tyrannine was remarkably increased, and the site and characteristics of the mutation were confirmed through the nucleotide sequence analysis of the secondary ultraviolet treated strain, Can be used as a biological control agent through the improvement of strains that are capable of producing the tyrins by using the tyrosine biosynthetic gene derived from E. coli and can be used for the development of new strains by transforming the genes into bacteria exhibiting other biological control activity have.