Properties of enzyme production of a Bacillus subtitles strain( MSJ-5) isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. β-1,4-D-mannanase was the main component of crude enzyme solution,test results showed that the optimal reaction temperature of the enzyme was 50 ℃,the optimum reaction p H was 7. 0,and the enzyme was stable when p H was 5. 0- 7. 0. Hydrolysis test results showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan,and the major hydrolysis product was mannan oligosaccharide. The results indicated that β-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligosaccharides industry.
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