齐整小核菌SCL2010产小核菌多糖培养基优化的研究

摘要

The medium components of high scleroglucan-producing Sclerotium rolfsii SCL2010 were deeply screened and optimized by single factor and orthogonal experiments.The results showed that the optimum medium formula was determined as follows:glucose 45.0 g/L,corn steep liquor 1.5 g/L,NaNO3 2.0 g/L,K2HPO4·3H2O 2.0 g/L,citric acid 0.7 g/L,MgSO4·7H2O 1.5 g/L,KCl 2.0 g/L.Under the optimal conditions,the yield of scleroglucan was 31.81 g/L,and the conversion rate of carbon source was 70.69％.Finally,the scale-up experiment was carried out by the fermentor,the yield of scleroglucan was 31.86 g/L and the conversion rate of carbon source was 70.80％,the apparent viscosity of fermentation broth reached 4 500 mPa·s,and the fermentation time was shortened to about 60 h,which had significant breakthrough.%采用单因素和正交试验对小核菌多糖高产菌株齐整小核菌(Sclerotium rolfsii) SCL2010的培养基成分进行了深入地筛选与优化.结果表明,最佳培养基配方为葡萄糖45.0 g/L,玉米浆1.5 g/L,NaNO32.0g/L,K2HPO4·3H2O 2.0 g/L,柠檬酸0.7 g/L,MgSO4·7H2O1.5 g/L,KCl 2.0 g/L.在此优化条件下,小核菌多糖的产量为31.81 g/L,碳源转化率为70.69％.采用发酵罐进行小试放大试验,小核菌多糖的产量达到31.86 g/L,碳源转化率为70.80％,发酵液表观黏度达到4 500 mPa·s,并将发酵时间缩短至60h左右,具有显著效果.