SLE结合GC-MS法测定葡萄酒中氨基甲酸乙酯

摘要

建立了固液萃取(SLE)结合气相色谱-质谱联用(GC-MS)法测定葡萄酒中氨基甲酸乙酯(EC)含量的检测方法.以硅藻土为吸附剂,二氯甲烷为萃取溶剂,对葡萄酒中氨基甲酸乙酯进行萃取.结果表明,二氯甲烷用量为70 mL,酒样中乙醇体积分数低于12％,萃取效果最佳.EC在200～1 600 μg/L范围内线性关系良好(R=0.997 9).加标回收率为91.30％～99.90％,相对标准偏差(RSD)为1.26％～1.98％,方法的精密度RSD值为1.61％～3.06％.表明该方法准确度高、重现性和精密度良好,适合用于葡萄酒样品中EC含量的检测.%The determination method of ethyl carbamate (EC) in wine was established by SLE combined with GC-MS.Using diatomite as adsorbent,dichloromethane as extraction solution,the EC in wine was extracted.The results showed that the optimum extraction conditions were as follow:dichloromethane dosage 70 ml,ethanol content in wine ＜ 12％.EC had a good linear relationship in range of 200-1 600 μg/L (R2=0.997 9),the adding standard recovery rate was 91.30％-99.90％,and relative standard deviation (RSD) was 1.26％-1.98％.The RSD of method precision was 1.61％-3.06％.Due to high accurate,good reproducibility and accuracy,the method could be used for the determination of EC content in wine.