复合诱变法筛选达托霉素高产菌株

摘要

From original strain of Sterptomyces roseosporus HK402,a high daptomycin-producing strain 14-10-98 was obtained by complex mutagenesis of He-Ne laser irradiation and NTG.The strain 14-10-98 had good genetic stability and the fermentation unit of daptomycin increased by 35% compared with the original strain,by 8% compared with the strain 14-8-102 screened by He-Ne laser irradiation alone,by 13% compared with the strain 14-9-196 screened by NTG mutagenesis alone.%以达托霉素产生菌 HK402为出发菌株进行氦氖激光辐照-亚硝基胍复合诱变，最终获得一株达托霉素高产菌株14-10-98。该菌株遗传稳定性好，发酵单位比出发菌株高35％，比单用氦氖激光辐照诱变得到的高产菌株14-8-102高8％，比单用亚硝基胍诱变得到的高产菌株14-9-196高13％。