Development and validation of polar RP-HPLC method for screening for ectoine high-yield strains in marine bacteria with green chemistry

摘要

A novel, green, rapid, and precise polar RP-HPLC method has been successfully developed and screened for ectoine high-yield strain in marine bacteria. Ectoine is a polar and extremely useful solute which allows microorganisms to survive in extreme environmental salinity. This paper describes a polar-HPLC method employed polar RP-C18 (5 urn, 250 x 4.6 mm) using pure water as the mobile phase and a column temperature of 30 °C, coupled with a flow rate at 1.0 ml7 min and detected under a UV detector at wavelength of 210 nm. Our method validation demonstrates excellent linearity (ft2 = 0.9993), accuracy (100.55%), and a limit of detection LOQ and LOD of 0.372 and 0.123 mugmL~(-1) respectively. These results clearly indicate that the developed polar RP-HPLC method for the separation and determination of ectoine is superior to earlier protocols.