尿素包合法富集鱼油乙酯中的EPA和DHA

摘要

采用尿素包合法富集鱼油乙酯中的二十碳五烯酸(EPA)和二十二碳六烯酸(DHA),通过单因素实验和正交实验确定最优富集工艺为：m(尿素)∶m(鱼油乙酯)为1.5∶1、V (乙醇)∶m(鱼油乙酯)为3∶1、包合时间1 h、包合温度75℃、结晶温度25℃,一次包合的 EPA+DHA含量可达85%以上,收率60%。%Eicosapentaenoic acid(EPA)and decosahexaenoic acid(DHA)from fish oil ethyl ester were en-riched by an urea inclusion method.The optimum enrichment process obtained by a single factor experiment and an orthogonal experiment were as follows:m(urea)∶m(fish oil ethyl ester)of 1 .5∶1 ,V(ethanol)∶m(fish oil ethyl ester)of 3∶1,inclusion time of 1 h,inclusion temperature of 75 ℃,crystallization temperature of 25 ℃. Under above conditions,the total content of EPA and DHA from fish oil ethyl ester reached 85%,and the yield was 6 0%.