高效液相色谱法测定婴幼儿乳制品中免疫球蛋白IgG的含量

摘要

A method for determining the IgG content of immune globulin in the infant dairy proucts accurately has been established in This topic. The research aims at the pre-treatment methods and high performance liquid chromatography separation detection conditions. The extraction solvent using in the sample pre-treatment、centrifu-gation rate、 ultrasonic time、sample dilution factor、the acid adjusting the pH and the detection wavelength、column flow rate、 column temperature and the pH of the mobile phase are discussed respectively, and adopting the retention time to determine the nature ,using the peak area external standard method to quantify. The detection limit of the method is 0.7μg·mL-1, the average recovery is 92.16%, the standard deviation is 3.34%, IgG content showed a good linear relationship with the target peak area in the 0.l~l.Omg·mL-1: y=2519x-24.71 (R2=0.9992). And with this method for analyzing the content of IgG in the actual samples. Also proved that the method was better than the chromatographic peak of national standard.%本文建立了一种测定婴幼儿乳制品中免疫球蛋白IgG含量的方法.针对前处理方法及高效液相色谱条件进行实验研究,确定了提取溶剂、离心速率、超声时间、样品稀释倍数、调pH值所用酸及检测波长、色谱柱、流速、柱温、流动相的pH值的参数值,采用保留时间定性,峰面积外标法定量.方法检出限为0.7μg·mL-1,回收率为92.16％,标准偏差3.34％,IgG浓度在0.1～1.0mg· mL-1范围内与其色谱峰面积之间为线性关系:y=2519x-24.71 (R2=0.9992).对实际样品中的IgG含量进行了测定.本文方法所获得的色谱峰分离度优于国标方法.