An analytical method for the determination of Benzo(α)pyrene in seafood was developed by isotope dilution gas chromatography tandem mass spectrometry(GC-MS / MS). Sample was extracted by using acetonitrile-acetone mixture as solvent, purged by silica solid phase extraction column and analyzed by GC-MS/MS under selective reaction monitoring mode. Three deuterated surrogate standards Benzo(α)pyrene-D12 were used as internal standards for quantification and analytical quality control. The limit of quantification(LOQs) was 0.2μg / kg, the recoveries ranged from 93% to 98%, and the relative standard deviations were 6.2%-12.2%(n=6). The method was used to determine the quality control materials T0642 provided by the FAPAS and the results were consistent with standard values.%采用同位素稀释法结合固相萃取净化,建立了海产品中苯并(a)芘残留的气相色谱串联质谱(GC-MS/MS)检测方法.样品经乙腈-丙酮(体积比6∶4)溶液提取,硅胶固相萃取净化,苯并(a)芘用GC-MS/MS测定,同位素D12-苯并芘内标法定量.方法的平均回收率为93％～98％,相对标准偏差为6.2％～ 12.2％(n=6),定量限为0.2μg/kg.用该方法测定FAPAS烟熏鱼有证标准样品,测定值与标准值一致.
展开▼
