免疫亲和柱净化-柱后衍生高效液相色谱荧光法测定食用油中黄曲霉毒素B1

摘要

样品经甲醇–水（7∶3，V/V）提取，提取液经过滤、稀释、免疫亲和柱净化，通过高效液相色谱柱后衍生法测定黄曲霉毒素B1的含量。实验结果表明，黄曲霉毒素B1在0.5~15.0 ng/mL范围内线性关系良好，相关系数为0.999。同一份样品加标后经三个不同品牌的免疫亲和柱纯化后，测定结果存在一定差异。不同样品中黄曲霉毒素B1加标回收率在87.2%~95.8%之间，相对标准偏差（n=6）在2.12%~6.51%之间。%The samples were extracted bymethanol–water solution(7∶3,V/V),then the extracted liquor was filtered,diluted with water and purified by immunoaffinitycolumn.TheAflatoxin B1 was determined byHPLC with post–column derivatization.The results showed that themethod had good linear relationship in the range of 0.5~15.0 ng/mL and thecorrelationcoefficients was 0.999.The measurement results had some differences from the same sample which was purified by three different brands of immunoaffinitycolumn. In different samples withAflatoxin B1,the recoveries ranged from 87.2% to 95.8% and the relative standard deviations(n=6) ranged from 2.12% to 6.51%.