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IMMUNOADSORBENT FOR PURIFYING FIVE KINDS OF MYCOTOXINS INCLUDING FUMONISIN B1 AND AFLATOXIN B1, AND COMPLEX AFFINITY COLUMN
IMMUNOADSORBENT FOR PURIFYING FIVE KINDS OF MYCOTOXINS INCLUDING FUMONISIN B1 AND AFLATOXIN B1, AND COMPLEX AFFINITY COLUMN
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机译:用于纯化五种霉菌毒素的免疫吸附剂,包括伏马毒素B1和黄曲霉毒素B1,以及复杂亲和力柱
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摘要
The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B1 and aflatoxin B1, and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B1 monoclonal antibody, an anti-aflatoxin B1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B1 sample, an aflatoxin B1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.
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