OBJECTIVE: To study the correlation between telomere length of peripheral blood leukocytes and noise-induced hearing loss. METHODS:In a factory that produced computer shells,all posts that had noise intensity LEX,8 h≥85 dB(A),as determined by SV104IS wireless noise intensity detector,were selected. Questionnaires were used for collecting general and occupational information from workers and real-time polymerase chain reaction was used to detect telomere length of their peripheral blood leukocytes. Ordinal ploytomous logistic regression was used for analyzing the correlation between telomere length and noise-induced hearing loss. RESULTS:The average noise intensity among LEX,8 h of overproof posts in the factory was (91.30±3.22) dB(A). This study recruited 127 noise-exposed workers consisting of 43 normal hearing workers (33.86%),75 abnormal high-frequency hearing loss workers (59.06%),9 abnormal speech-frequency hearing loss accompanied with abnormal high-frequency hearing loss (7.09%). Statistical significance was found in relative telomere length between different levels of noise-induced hearing loss (P<0.05). It was revealed that noise intensity,age,gender and relative telomere length were factors related to noise-induced hearing loss (P<0.05). CONCLUSION:Relative telomere length of peripheral blood leukocytes can potentially be used as a biomarker of susceptibility for noise-induced hearing loss.%目的:探讨外周血白细胞端粒相对长度与噪声性听力损失(NIHL)的相关性.方法:用SV104IS无线个体噪声剂量计对某电脑机箱外壳制造企业所有作业岗位进行噪声强度检测,选择噪声强度超标[L≥85 dB(A)]生产岗位的全体生产工人作为研究对EX,8 h象.采用问卷调查法获得研究对象的一般情况和职业史,实时荧光定量PCR检测研究对象外周血白细胞端粒相对长度,有序多分类Logistic回归法分析外周血白细胞端粒相对长度与噪声性听力损失(NIHL)的相关性.结果:该企业噪声超标作业岗位噪声强度(L)均值为(91.30±3.22)dB(A).本次研究共调查127名噪声作业者:双耳听力正常43人(33.86%),双耳高频听阈提高75人EX,8 h(59.06%),单耳或双耳语频听阈提高伴双耳高频提高9人(7.09%).不同NIHL程度工人的端粒相对长度差异有统计学意义(P<0.05).有序多分类Logistic回归分析显示,噪声强度、年龄、端粒相对长度、性别是NIHL的影响因素(P<0.05).结论:外周血白细胞端粒长度可能是发生NIHL的易感性生物标志物.
展开▼
