一种具有琼胶酶活性的海洋细菌淀粉酶的表达和分析

摘要

Our aim is to confirm that there is amylase with agarase activity in the novel marine bacterial strain ZC1.The amylase gere amy440 of strain ZC1 was cloned and expressed in recombinant prokaryotic vector and the amylase and agarase activity of the recombinant protein was determined using the method of activity staining electrophoresis.Results indicated that the recombinant protein of gene amy440 showed amylase and agarase activity in the activity staining electrophoresis.Conclusively,the encoded protein of gene amy440 of strain ZC 1 is an amylase with agarase activity.%旨在验证海洋新种细菌ZC1中可能存在的具有琼胶酶活性的淀粉酶.对菌株ZC1中的淀粉酶编码基因amy440进行基因克隆和重组原核表达,并用活性电泳法验证重组蛋白的淀粉酶和琼胶酶活性.结果显示,基因amy440的重组蛋白在淀粉酶和琼胶酶活性电泳上均出现活性显色条带.菌株ZC1中基因amy440的编码蛋白是一种具有琼胶酶活性的淀粉酶.