发酵乳酸杆菌对肉鸡血清抗氧化性能及肠道挥发性脂肪酸（VFA）的影响

摘要

旨在研究添加不同剂量的发酵乳酸杆菌F-6对肉鸡血清抗氧化性能及肠道VFA含量的影响。选择健康的1日龄AA肉仔鸡240只，称重后随机分为4组，各组肉仔鸡的初始体重差异均不显著（P>0.05）。各组肉仔鸡基础日粮一致，3个试验组在饮水中添加发酵乳酸杆菌F-6，添加剂量分别为2.0×105、2.0×106、1.0×107 CFU/mL，对照组肉仔鸡不饲喂发酵乳酸杆菌。于21日龄及42日龄时，分别测定各组肉鸡血清中的MDA和SOD含量，以及肠道中VFA含量。结果表明：21日龄时，试验3组肉鸡血清中的SOD含量显著高于对照组（P<0.05）；42日龄时，试验2组和3组肉鸡血清中的SOD含量也显著高于对照组（P<0.05）。21日龄和42日龄时，各试验组肉鸡盲肠及回肠中的乙酸和总挥发性脂肪酸（TVFA）含量均显著高于对照组（P<0.05）；除试验1组外，其他各试验组肉鸡的回肠及盲肠丁酸含量均显著高于对照组（P<0.05）。综上提示，发酵乳酸杆菌F-6能够显著提高肉鸡的血清抗氧化性能及肠道部分VFA含量，其具有开发为新型饲料益生菌添加剂的应用前景。%This experiment was conducted to assess the supplementation of Lactobacillus fermentum F-6 with different levels in basal diet on serum antioxidant capacity and volatile fatty acid (VFA) in intestine of broiler chickens. A total of 240 1-day-old healthy AA broiler chickens were included in this study and were randomly divided into 4 groups (3 experimental groups and 1 control group). No significant differences were observed in initial body weight between the chickens of different groups (P>0.05). The same basal diet was supplied for each group;however, the basal diet of the three experimental groups was supplemented with 2.0×105, 2.0×106 and 1.0×107 CFU/mL of Lactobacillus fermentum F-6, respectively. No additions of Lactobacillus fermentum F-6 were made in basal diet of the control group. The serum content of MDA and SOD as well as content of VFA in intestine of 21-day-old and 42-day-old broiler chickens in each group were determined. The results showed that the serum content of SOD of 21-day-old chickens of experimental group 3 was significantly higher than that of control group (P<0.05);the serum content of SOD of 42-day-old chickens in experimental group 2 and 3 were significantly higher than that in control group (P<0.05);the content of acetic acid and total volatile fatty acid (TVFA) in cecum and ileum of 21-day-old and 42-day-old broiler chickens of all experimental groups were significantly higher than that in control group (P<0.05);except for experimental group 1, the content of butyrate in cecum and ileum of 21-day-old and 42-day-old broiler chickens of the other experimental groups were significantly higher than that of control group (P<0.05). It was demonstrated that the supplementation of Lactobacillus fermentum F-6 in basal diet was able to improve the serum antioxidant capacity and increase the level of VFA in intestine of broiler chickens, which was potential to be developed as a new type of additive.