普洱茶茶多糖的提取工艺的响应面分析研究

摘要

This study aimed to optimize the extraction process parameters of Pu-erh tea polysaccharide.[Method] Single-factor experiment was carried out to analyze the influences of three main factors,including extraction temperature,extraction duration and solid-liquid ratio,on the extraction yield of tea polysaccharide.Box-Behnken central composite design and response surface methodology were adopted to determine the optimal extraction process of Pu-erh tea polysaccharide.[Result] The results of response surface analysis showed that the optimal extraction process was solid-liquid ratio of 1∶17,extraction temperature of 80 ℃ and extraction duration of 78.5 min,and the Pu-erh tea polysaccharide yield was 12.72％.[Conclusion] Using response surface methodology (RSM) is feasible for the optimization of Pu-erh tea polysaccharide extraction process,and the tea polysaccharide yield increased significantly.%[目的]该研究旨在解决普洱茶茶多糖的提取工艺中参数设定的问题.[方法]采用单因素试验分析浸提温度、浸提时间、料液比这3种主要因素对茶多糖提取率的影响,利用Box-Behnken中心组合试验和响应面分析法,确定了普洱茶茶多糖的最佳提取工艺.[结果]响应面法优化提取工艺为:料液比为1∶17,浸提温度为80℃,浸提时间为78.5 min,茶多糖得率为12.72％.[结论]采用响应面法分析法对普洱茶茶多糖提取工艺进行优化可行,茶多糖的提取率增加明显.