水稻SDG711蛋白C末端原核表达及多克隆抗体制备

摘要

[目的]对水稻SDG711蛋白C末端进行原核表达,并制备其多克隆抗体.[方法]选取水稻SDG711蛋白抗原决定簇较密集的C末端进行原核表达,通过构建原核表达载体pET28a-711C,转化E.coli BL21 (DE3)感受态细胞,IPTG诱导表达融合蛋白后进行纯化,再以纯化的融合蛋白为抗原免疫新西兰白兔,制备多克隆抗体,并对其进行Western-blot分析.[结果]试验制备的多克隆抗体能有效地检测抗原的表达.[结论]该研究为进一步深入研究SDG711蛋白的功能奠定了基础.%[Objective] This study aimed to conduct prokaryotic expression of rice SDG711 C-terminal and to prepare its polyclonal antibody.[Method] C-terminal of rice SDG711 containing relatively intensive antigen determinants was selected for prokaryotic expression,prokaryotic expression vector pET28a-711C was constructed and the recombinant plasmid was transformed into Escherichia coli BL21 (DE3) competent cells.The recombinant fusion protein was induced by IPTG and purified to immunize a New Zealand white rabbit as the antigen,and polyclonal antibody was obtained and confirmed by Western-blot analysis.[Result] The polyclonal antibody was successfully prepared and could efficiently detect the expressed antigen.[Conclusion] This study laid the foundation for further investigating the functions of SDG711 protein in rice.