一种检测污染水体致突变性的快速简便方法

摘要

采用鼠伤寒沙门氏菌TA98菌株为测试菌株,二氨基芴为阳性物,通过分析菌液体积分数、S9体积分数、阳性物质量浓度的配比关系,确定了用于污染水体致突变性检测的微量波动法的最佳反应条件.在最佳反应条件下测试TA97、TA100、TA102菌株在河流水样检测中的反应情况,确定了适用于鼠伤寒沙门氏菌系列菌株在污水检测中的反应条件.%The best reaction condition of microtitre fluctuation test was obtained in polluted water to detect mutagenicity by analyzing bacteria liquid and S9 volume fraction using Salmonella typhimurium TA98 as test strain, and diaminofluorene as positive reactor. By using the best reaction condition, response of strain TA97, TA100, TA102 in polluted water had been analyzed to establish suitable reaction conditions for Salmonella typhimurium in detecting the mutagenicity.