Method for rapid detection and identification of one or more target microorganisms living in a sample; a device for rapid detection and identification of one or more target microorganisms living in a.Oyster; and test kit for rapid detection and identification of one or more target microorganisms living in a sample for use in the method.

摘要

Summary method for rapid detection and identification of one or more target microorganisms living in a sample; a device for rapid detection and identification of one or more target microorganisms living in aA sample; and test kit for rapid detection and identification of one or more target microorganisms living in a sample for use in the method of the invention provides methods.Devices and kits for rapid detection and identification of one or more target microorganisms living in a sample liquid or developed in plates containing nutrient media solids.The invention includes mixing one or more microorganisms targets with one or more aptamers and / or one or more antibodies.Each compound conjugated to a reporter and specific to a first location in one or more microorganisms targets to form a mixture.The mixture is placed in a permeable membrane having immobilized therein one or more aptamers linked to an amine compound, and / or one or more antibodies.Each specific to a second location in one or more microorganisms targets or a place in aptu00e2mero conjugate and / or conjugated antibody.A detection solution is added to the membrane and the detection and identification of one or more microorganisms targets is obtained in approximately an hour or less.