基于高密度SSR连锁群的烟草致香物质关联分析

摘要

Eleven tobacco varieties with different aroma types were selected as samples to examine 52 aroma constituents by Gas Chromatography. 62 simple-sequence repeat (SSR) markers which come from a high density tobacco SSR linkage group were scanned. Marker-trait association analysis showed that there were 24 loci significantly associated with 17 types of aroma constituents and each locus was often associated with several aroma constituents. LD analysis revealed two haplotypes at this linkage group, which were tsHap1_1 and tsHap1_2. The former consisted of locus 52429 and 53840 while the latter comprised locus 61443, 52305 and 55052, occupying 3.012cM. 9 aroma constituents were found to be associated with tsHap1_2. Therefore, the associated SSR loci and haplotype with aroma constituents are both beneficial for marker assistant selection in tobacco leaf quality improvement.%烟叶致香物质是烤烟品质及香型风格的物质基础，是烟草品质改良的重点之一。采用气相色谱方法，对11份不同香型风格的烟草种质进行了52种致香成分的检测，同时采用1条高密度连锁群上的62个SSR位点对其进行了分子标记位点分型。通过标记-性状关联分析，发掘到17种烟草致香物质的24个关联位点。大多数位点关联到了多种致香成分。对供试材料的连锁不平衡分析，发现该连锁群存在2个单倍型，单倍型tsHap1_1由位点52429和53840组成，未发现其与致香物质相关联；单倍型tsHap1_2由3个相邻的位点61443、52305和55052组成，总长度3.012cM，研究发现该单倍型与9种致香物质关联。本研究发掘的关联位点和单倍型，可用于烟草高香气育种分子标记辅助选择。