The F2 population derived from crossing sorghum cultivars'B2V4'and'1383-2'was used as research material,with the SSR and MSAP markers used to detect the sorghum genome differences and construct methylation linkage groups. Results showed that the methylation linkage group LGC covered 44.3 cM,of which 23 loci were MSAP markers and three loci were SSR markers; the methylation linkage group LGD covered 46.2 cM,of which eight loci were MSAP markers and four loci were SSR markers. The methylation sites on linkage group LGC were from EcoRⅠ/MspⅠ enzyme digestion,but the methylation sites on linkage group LGD were from EcoRⅠ/Msp Ⅰ and EcoRⅠ/Hpa Ⅱ enzyme digestions. A dense methylation region near Xtxp 69 in LGC was identified. The MSAP marker rapidly detected genomic methylation differences and was able to construct a methylation linkage group in plants.%以高粱(Sorghum bicolor (L.) Moench)品种'B2V4'和'1383-2'杂交获得的F2群体为材料,通过SSR和MSAP标记检测高粱基因组差异,构建其甲基化遗传连锁群.结果显示,高粱甲基化连锁群LGC含有3个SSR标记和23个甲基化标记,覆盖高粱基因组44.3 cM;甲基化连锁群LGD含有4个SSR标记和8个甲基化标记,覆盖高粱基因组46.2 cM.LGC上甲基化位点仅来源于EcoRⅠ/MspⅠ酶切组合,而LGD上有来源于EcoRⅠ/MspⅠ和EcoRⅠ/HpaⅡ两种酶切组合的甲基化位点.在LGC连锁群Xtxp69附近检测到一个密集的甲基化位点区域.研究结果表明MSAP标记可以快速检测植物基因组甲基化差异,适用于构建甲基化连锁群.
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