罗氟司特对百草枯诱导的小鼠肺、肝、肾组织损伤与纤维化的抑制作用

摘要

为研究罗氟司特对百草枯（paraquat，PQ）中毒诱导的小鼠肺、肝、肾等器官组织损伤与纤维化的抑制作用，用昆明种小鼠腹腔注射 PQ 20 mg/kg造模，给药组每天灌胃5 mg/kg地塞米松或罗氟司特，共给药21 d，造模结束后手术分离小鼠肺、肝、肾等器官，进行HE染色和Masson染色分析，观察肺、肝、肾等器官组织损伤和纤维化变化。结果发现腹腔注射PQ 20 mg/kg显著诱导了小鼠肺、肝、肾等器官的组织损伤与纤维化病变。罗氟司特组小鼠每天给予罗氟司特5 mg/kg持续21 d后，PQ诱导的小鼠肺、肝、肾组织损伤与纤维化程度均明显减轻。实验结果表明罗氟司特对PQ诱导的小鼠肺、肝、肾等器官组织损伤与纤维化具有明显的抑制作用。%The aim of the present study is to verify whether roflumilast attenuates PQ-induced injury and fibrosis in lung,liver and kidney tissues of mice.Mice are pretreated with PQ of 20 mg/kg (i.p.),and then dealt with roflumilast or dexamethasone 5 mg/(kg·d)for 21 days.The tissues of lung,liver and kidney are surgically separated and immersed in 1 0%neutral formalin.The injury and fibrosis in tissues of lung,liver and kidney are observed by H&E staining and Masson staining.The results show that PQ (20 mg/kg,i.p.)induces remarkable toxicity and fibrosis in tissues of lung,liver and kidney.Roflumi-last (5 mg/kg per day for 21 days)treatment reduces PQ-induced injury and fibrosis in tissues of lung, liver and kidney,which is superior to dexamethasone.So,roflumilast attenuates PQ-induced injury and fibrosis in tissues of lung,liver and kidney of mice.