Under the condition of physiological environment at pH 7.4, the binding ratio nγ-CD:nAYR = 1:1 and binding constant Kθf,288.15K= 1. 69×105 L/mol were confirmed by mole ratio method and linear method. AO was used as molecule probe, and the interaction between γ-CD-AYR and herring-sperm DNA was studied by UV spectra, fluorescence spectra, thermodynamic method and viscosity method, etc. A series of thermodynamic data such as △rHmθ = 8. 78×103J/mol, △rGmθ= - 2. 59×104 J/mol, △rSmθ= 116. 45 J/ (mol · K) were obtained, and △rSmθ was the primary driven power of this interaction, the binding ratio was nDNA:nγ-CD-AYR=1:2, the binding constants were K288.15K = 3. 10×104 L/mol, K310.15K = 4. 02×104 L/mol. The interaction mode between γ-CD-AYR and herring-sperm DNA was an electrostatic interaction and part embedded intercalation.%在生理pH =7.4环境下,用摩尔比法测定γ-环糊精(γ-CD)与茜素黄R(AYR)的包合比(摩尔比)nγ-CD:nAYR=1:1,确定包合常数K(θ)∫,288.15K=1.69×105 L/mol.以吖啶橙(AO)作为分子探针,用紫外光谱法、荧光光谱法、化学热力学法和黏度法等研究包合物γ-CD-AYR与鲱鱼精DNA(hsDNA)的作用,得到γ-CD-AYR包合物与hsDNA作用的结合比nDNA:nγ-CD-AYR=1:2,结合常数为K288.15K=3.10×104L/mol,K31015K=4.02×104 L/mol.热力学函数△rHm(θ)=8.78×103J/mol,△rGm(θ)=-2.59×104 J/mol,△rSm(θ)=116.45 J/(mol·K),说明γ-CD-AYR包合物与DNA作用为熵驱动.确定γ-CD-AYR与hsDNA的作用方式为静电和部分嵌插的作用方式.
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