脂质体对草鱼出血病核酸疫苗免疫效果的影响

摘要

Recombinant plasmids pFastBac-β-VP71-VP72, which were used as DNA vaccines based on 30 μg dose, were injected into grass carps by dorsal muscles after being enwrapped by liposome. Transcription situation and antibody level were tested respectively on a regular basis from RT-PCR and indirect agglutination reaction for evaluating the role of liposome in comparison with no liposome groups, pFastBacTMDual group and control. The results indicated that transcription of target gene was still in process on the 70th day in liposome group, and antibody level was higher than no liposome groups. Therefore, liposome could promote the immune effect of DNA vaccine and should be further studied and put into practice as a kind of DNA vaccine adjuvant.%将已构建完成的草鱼出血病核酸疫苗pFastBac-β-VP71-VP72以30μg的剂量,经Lipotap脂质体包裹后,背部肌肉注射草鱼体内,定期通过RT-PCR测定目的基因转录水平、间接凝集反应测定抗体水平,并通过与相应的重组质粒组、空载体组和对照组进行比较,来评价脂质体对核酸疫苗免疫保护效果的影响.结果显示,脂质体组至第70天仍能检测到目的基因的转录,且抗体效价明显高于其他各组,说明脂质体可显著增强该核酸疫苗的免疫活性,可作为一种有应用前景的核酸疫苗佐剂.