结球甘蓝体细胞无性系变异的RAPD分析

摘要

In this experiment,protoplast regenerated plants and no vaccine hypocotyl regenerated plants of cabbage (Brasssica oleracea L.var capitata) were used as materials,using RAPD molecular markers to analyze the somaclonal variation in cabbage regeneration process.The results were as follows:14 primers which were screened out of 50 primers,using which 253 repeatable loci were amplified including 73 polymorphic loci.This polymorphic loci showed absence of DNA fragments and newly amplified bands.The percentage of polymorphic bands was 28.85％.Each primer produced 5.21 polymorphic bands on average.According to RAPD molecular markers,genetic similarity of 17 varieties of cabbage were computed and cluster analysis diagram was drawn using UPGMA.The results showed that:the average genetic similarity was 0.9304,the genetic variation exists in regenerated plants.%以结球甘蓝强力50原生质体再生植株和无菌苗下胚轴再生植株为材料,利用RAPD分子标记技术对结球甘蓝再生过程中产生的体细胞无性系变异进行了分析检测.结果表明:从50条随机引物中筛选出条带清晰、多态性丰富、表现稳定的引物14条,在此基础上共扩增出DNA片段253条,其中具多态性的73条,表现为新增加带和缺失带,多态率为28.85％,每对引物平均扩增的多态位点为5.21个.各植株间遗传相似系数的计算使用聚类分析软件NTSYS2.10e完成,采用非加权类平均法(UPMGA)对统计数据进行聚类分析,结果表明:各再生植株与母本间平均遗传相似系数为0.9304,植株之间存在遗传变异.