黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响

摘要

旨在探讨黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响.新城疫病毒(NDV)感染鸡胚成纤维细胞,使用MTT法检测黄芩甙对鸡胚成纤维细胞的最大安全浓度,使用荧光定量PCR方法检测不同浓度黄芩甙对染毒细胞Toll样受体2、3、4、7 mRNA在不同时间表达的影响.结果表明,黄芩甙组和病毒对照组细胞TLR2、TLR4和TLR7 mRNA的表达水平起初受到抑制,TLR2和TLR4 mRNA在24 h内呈上升趋势,TLR7 mRNA在16 h内呈上升趋势,TLR3 mRNA的表达水平呈波动性变化.黄芩甙在24 h时能够促进NDV感染细胞TLR2和TLR4 mRNA的表达,且呈剂量依赖性,从而发挥免疫调节作用,具有抗NDV的功能.%The purpose of this study was to investigate the changes of the expression of TLR2, TLR3, TLR4 and TLR7 gene in NDV-infected cells and the effect of Baicalin on them .NDV infection of chicken embryo fibroblasts (CEFs) was performed.Cytotoxicity of Baicalin was tested by the MTT method .Quantitative expressions of TLR2, TLR3, TLR4 and TLR7 gene were analyzed by real-time fluorescence quantitative PCR (RTFQ PCR).The results showed that the expressions of TLR2, TLR4 and TLR7 genes were suppressed by NDV in the early infection stage , while their expression were enhanced with the replication of NDV and the expression of TLR2 and TLR4 gene reached the peak level at 24 h.As well as the expression of TLR7 gene reached the peak level at 16 h.The infection of NDV could change the expression of TLR3 gene, but it was not regular .Besides, Baicalin could increase the expression of TLR2 and TLR4 genes in NDV-infected cells and show the dosage-dependence , which enhanced immune function and possessed anti-NDV activity .