复方中药制剂对SD大鼠血清抗氧化功能的影响

摘要

由黄芪、板蓝根、炒白术、茯苓、陈皮、山楂、甘草和山药等中药制成的复方中药制剂,研究其对大鼠血清总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的影响.选择150只断奶SD大鼠(SPF级)分为对照组、1.5 mL·kg-1组、3.0 mL·kg-1组和6.0 mL·kg-1组,对照组有15只大鼠,其余试验组各有45只大鼠.试验期间3个试验组大鼠按每千克体质量每天分别灌胃1.5、3.0和6.0 mL复方中药制剂(含生药1 g·mL-1),对照组灌胃3.0 mL蒸馏水.于试验开始后10、30、60 d,每组分别取5只大鼠禁食禁水10 h后称重、心脏采血,分离血清,测定样本血清的T-AOC、T-SOD、GSH-Px活性和MDA含量.结果表明,3.0 mL·kg-1组和6.0 mL·kg-1组在灌胃后10 、30 和60 d,其血清T-AOC活性与同日期对照组比较有显著(P<0.05)或极显著(P<0.01)增加;而血清中MDA含量显著(P<0.05)或极显著(P<0.01)减少.3.0 mL·kg-1组在灌胃30 和60 d,血清T-SOD活性与同日龄对照组比较显著增加(P<0.05);6.0 mL·kg-1组在灌胃30 和60 d,血清GSH-Px活性与同日龄对照组比较显著增加(P<0.05).说明口服以黄芪、板蓝根、炒白术等药材组成的复方中药制剂可显著提高SD大鼠的抗氧化功能,其对大鼠的最适灌胃剂量为3.0 mL·kg-1.%To study the effects of compound traditional Chinese medicine preparation, which consists of Radix astragali, Radix isatidis, Fried atractylodes, Poria cocos, orange peel, hawthorn, liquorice and yam on the T-AOC, T-SOD, GSH-Px activity and MDA content in serum of SD rats.150 SD rats (SPF animal) were randomly divided into control group, 1.5, 3.0 and 6.0 mL·kg-1 treatment group with 15, 45, 45, 45 SD rats, respectively.The rats of different treatment groups were administered using gavage methods according to dosage 3.0 mL·kg-1 distilled water for control group, and 1.5, 3.0 and 6.0 mL·kg-1 compound traditional Chinese medicine preparation (containing raw herbs 1 g·mL-1) for treatment groups, respectively.Rats were regularly weighed and given medicine or distilled water by gavage every day.On the 10th, 30th and 60th day of the experiment, 5 rats fasted and banned water for 10 h were selected in every experiment group to be weighed and picked blood from the heart, for isolating serum sample to measure T-AOC, T-SOD, GSH-Px activities and MDA content.The results showed that on the experimental 10th, 30th and 60th day, the T-AOC activity in 3.0 mL·kg-1 and 6.0 mL·kg-1 group significantly (P<0.05) or extremely significantly increased (P<0.01), and the MDA contents in 3.0 mL·kg-1 and 6.0 mL·kg-1 group significantly (P<0.05) or extremely significantly decreased (P<0.01), compared with the control group on the same day.On the 30th and 60th day, the T-SOD activity in 3.0 mL·kg-1 group significantly increased (P<0.05), and the GSH-Px activity in 6.0 mL·kg-1 group significantly increased (P<0.05), compared with the control group.Compound traditional Chinese medicine preparation could significantly improve the SD rats` antioxidant function.The optimum dosage of compound traditional Chinese medicine preparation to rats was 3.0 mL·kg-1 by gavage methods.