香菇菌糠纤维素酶的提取工艺优化

摘要

Cellulase of the waste material from Lentinus edodes was prepared by ultrasonic extraction, the effects of ultrasonic power, ultrasonic time and liquid-solid ratio on the activity of cellulase were studied through single factor tests and response sur-face analysis.The results showed that the optimum process parameters were obtained as follows:ultrasonic power was 500 W, ul-trasonic time was 15 min, liquid ratio was 20∶1, three validation tests under these conditions , the activity of the extracted cellu-lase was 9.61 IU/g.%以香菇菌糠为原料，采用超声浸提法制备纤维素粗酶液，通过单因素试验和响应面设计，分析了超声功率、超声时间和液料比等因素对纤维素酶浸提的影响。结果表明，香菇菌糠纤维素酶最佳提取工艺为：超声功率500 W，超声时间15 min，液料比为20∶1，在此条件下的CMC酶活力为9．61 IU／g。