Anti-inflammatory Activity of Quercitrin on Hypoxia-induced EA.hy926

摘要

The evidence support the existence of oxygen deprivation involved in pregnancy disorder or preeclampsia. The intensive hypoxia also regulates production of placental proinflammatory cytokines. Quercitrin belongs to flavonoid group is known to have antioxidant and anti-inflammatory activity. This study aims to determine the potential of Quercitrin as antiinflammatory in hypoxia-induced EA.hy926 as preeclampsia model. The cytotoxic assay of Quercitrin against EA.hy926 was conducted using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The data were analyzed using SPSS ver 20.0 software. One-way analysis of variance (ANOVA) was conducted, followed Tukey HSD post-hoc test and p<0.05 was considered to be significant. The anti-inflammatory potential of Quercitrin (25 and 6.25 μg/ml) toward hypoxia-induced EA.hy926 was determined using ELISA to measure IL-10 and TNF-α. Quercitrin (3.13 - 25 μg/ml) were nontoxic to the EA.hy926 cells. Quercitrin (25 and 6.25 μg/ml) were capable to reduce TNF-α compared to positive control (2% O2 hypoxia-induced cells) but no significant increase in IL-10. Our study suggests that quercitrin possess anti-inflammatory properties through suppression of TNF-α in hypoxia-induced EA.hy926 cells.