Acetylcholinesterase Inhibitory Activities of Douchi. a Traditional Chinese Food

摘要

The effect of different extract conditions on acetylcholinesterase (AChE) inhibitory activities of Douchi were studied, The in vitro AChE inhibitory activity of 9 kinds of commercial Douchi were measuerd, expessed as IC_(50) . Ethanol extracts showed highest AChE inhibitory activity with IC_(50) value of 0. 023mg/mL, while water extracts did not show any inhibition. When extracted by ethanol, the IC_(50) of Douchi increased with the decrease of ethanol concentration and solid liquid ratio. No significant differences were observed within IC_(50) value of the Douchi extracts with different extract time. 80% ethanol extracts of these nine Douchi inhibited human acetylcholinesterase with IC_(50) value ranged from 0. 033 to 1. 110mg /mL. This novel finding suggested that Douchi contain anti-acetylcholinesterase components, which may be used to retard the progression of Alzheimer disease, and indicated the potential to develop new functional foods.