Isolation and Characterization of Active Components Derived from Whole Plant of Mucuna pruriens (Linn.)

摘要

The aim of the present investigation was isolation and characterization of active components derived from whole plant of Mucuna pruriens. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to be more active among them. The preliminary phyto-chemical results revealed that phytosterols, flavonoids and amino acids as active constituents in methanolic extract of Mucuna pruriens. The methanolic extract of Mucuna pruriens was undergone column chromatography with different solvent fractions. Despite, three compounds were isolated from methanolic extract of Mucuna pruriens with the compound 1 was eluted with benzene: Chloroform 90:10, v/v and compound 2 were eluted with eluted with ethyl acetate: methanol 80:20, v/v and then compound 3 were eluted with ethyl acetate: methanol, 70:30, v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as acetate of 3β-Hydroxy-5α-Cholanic acid (C_(26)H_(38)O_3), the compound 2 was characterized as 3, 5, 7, 4'-Tetrahydroxy-6-methoxyflavone (C_(16)H_(12)O_7) and the compound 3 was characterized as Ethyl 2-amino-5-hydroxy-3, 6, 6-trimethyl heptonate (C_(12)H_(25)NO_3). Furthermore, pharmacological studies required for the isolated compounds.