Isolation and characterization of active constituent from sambucus chinensis, being a component for the treatment of epilepsy

摘要

A process for isolating and identifying the active constituent of chloroform extract of Sambucus chinensis, being a constituent for the treatment of Epilepsy, comprising the steps, in combination, of subjecting samples of the said extract to Column chromatography and Thin layer column chromatography for the separation of active constituents; Fourier transforms infrared (FTIR) spectroscopy for analysis of the phytoconstituents functional groups for identifying the functional groups; identification of the structure of the active constituents by Nuclear Magnetic Resonance (NMR) spectroscopy: two dimensional nuclear magnetic resonance (2D NMR) spectroscopy for resolving and identifying the nature of chemical sites in the sample; HSQC (Heteronuclear Single Quantum Coherence) for the detection of bond couplings; *H - *H COSY (Homonuclear Correlation Spectroscopy) for revealing correlations between coupled protons; the overall analysis indicating a structure of the active constituent of the said extract with a molecular formula = Ci6H3202, Molecular weight: 256.424 grams/mole and the Compound name: Hexadecanoic acid.