A Selective and Sensitive LC-MS/MS Method for the Determination of Beclomethasone Dipropionate and Beclomethasone-17-Propionate in Human Plasma

摘要

Beclomethasone dipropionate (BDP) is a topically active corticosteroid used in the treatment of asthma and rhinitis. In recent years, several bioanalytical methods have been developed to support pharmacokinetic (PK) analysis in clinical trials, with the most sensitive method having a lower limit of quantification (LLOQ) of 50 ph/mL for both BDP and B-17-P in human plasma. BDP is extensively metabolized in the liver after oral administration, resulting in very low plasma levels. To improve PK evaluation in clinical trials, we have developed an LC-MS/MS method with increased sensitivity compared with existing methods. The LLOQs were lowered to 10 and 20 pg/mL for BDP and B-17-P, respectively.