Label-free Relative Quantification of Co-eluting Isobaric Phosphopeptides of Insulin Receptor Substrate-1 by HPLC-ESI-MS/MS

摘要

We developed a label-free quantification approach for co-eluting isobaric phosphopeptides using unique fragment ions. This quantification strategywill prove useful for understanding how various cell stimuli regulate protein function by phosphorylation.