IN VIVO REAL-TIME MONITORING SYSTEM FOR METABOLITES IN A LIVING MOUSE BRAIN USING PROBE ELECTROSPRAY IONIZATION/TANDEM MASS SPECTROMETRY (PESI/MS/MS)

摘要

Recent improvements in ambient ionization techniques have achieved real-time monitoring of biogenic compounds in organisms. Here, we developed a novel analytical system for in vivo realtime monitoring of metabolites in a living mouse brain [1]. It consists of a manipulation system and a probe electrospray ionization unit, which uses an extremely thin solid needle (tip dia.: 700 nm) for direct sampling and ionization, coupled to a tandem mass spectrometer [1-3].