Quantitation of Four Chiral Drug Compounds Simultaneously Using Normal Phase LC-MS/MS Equipped With an APPI Ion Source

摘要

Under the optimized conditions, the chiral compounds were baseline separated and eluted at 5.4, 7.5, 8.3 and 11.4 min, respectively. The internal standard was also separated as two chiral forms, with RT at 9.0 and 11.1 min. It was demonstrated that better accuracy was obtained by applying the second IS for correcting the last chiral compound, while using the IS at 9.0 min for the first three chiral compounds. The LLOQ was 50 pg/mL for all four chiral compounds.