Lipid 654, a non-glycerol and non-phospholipid complex lipid of P. ging, can be hydrolyzed by either phospholipase A2 from porcine pancreatic or honey bee venom to yield Lipid 430 and isoC15:0 fatty acid. P. ging Lipid 654 has two groups of isoforms that can be identified using differential mobility separation. Only a fraction of one isoform group can undergo enzymatic hydrolysis and the enzyme-susceptible fraction appears to be the biologically active form of Lipid 654. Two atoms of ~(18)O can be added to the C15:0 fatty acid product. When using buffer containing ~(18)O-water, only iso-branched fatty acid, not anteiso-branched, can incorporate heavy oxygen via enzyme catalysis.
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机译：脂质654，一种非甘油和非磷脂复合物脂质的P.Ging，可以通过磷脂酶A2从猪胰腺或蜂蜜蜂毒液中水解，得到脂质430和ISOC15：0脂肪酸。 P. Ging Lipid 654具有两组同种型，可以使用差动迁移率分离来识别。只有一小部分的一个同种型组可以进行酶水解，并且酶易感部分似乎是生物活性形式的脂质654.〜（18）O的两个原子可以加入C15：0脂肪酸产物中。当使用含有〜（18）o水的缓冲液时，只有异化脂肪酸，而不是脱离分支，可以通过酶催化掺入重氧氧。
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