纤维质水解液发酵生产生物油脂

摘要

为提高发酵性丝孢酵母对纤维质水解液的适应性,对该菌株进行了驯化.利用驯化后的菌株发酵纤维质水解液生产生物油脂,并对纤维质水解液发酵体系进行优化.通过摇瓶培养试验,得到了优化体系.结果表明:驯化后的菌株发酵纤维质水解液的糖利用率比原来提高了37.2%,生物量提高了24.3%,油脂含量提高了18.3%,油脂浓度提高了45.6%.优化后的发酵条件为:初始糖浓度60～80 g/L,初始pH 6.O,质量比3:1的酵母粉+(NH4)2SO4复合氮源.在该条件下,可得菌体生物量、油脂含量和油脂浓度分别为22.6 g/L、46.3%和10.5 g/L.同时对其生物油脂的成分进行了分析.%Trichosporon fermentans (TF)was domesticated to improve adaptability in fiber hydrolysate.Fermentation system from fiber hydrolysate by the domesticated TF have been optimized.By the flask experiments of several single conditions which affect TF growth and fat synthesis, the optimal fermentation conditions were studied.After domestication,the transform rate of sugar increase 37.2％, the biomass increase 24.3％ ,lipid content increase18.3％ and lipid concentration increase 45.6％.The optimum fermentation conditions which were determined on the basis of a single test to be as follows:concentration of sugar,60 ～ 80 g/L, pH 6.0, quality ratio of yeast + (NH4)2SO4 3∶ 1.On the optimum condition, the biomass, lipid content and lipid concentration is 22.6 g/L, 46.3％ ,and 10.5 g/L, respectively.At the same time,the composition of bio-oil was analyzed.