玉米皮渣水解液高密度发酵生产微生物油脂的研究

摘要

The production of microbial oils with corn fiber hydrolysate using high density fermentation was studied. The conditions affecting the yield of microbial oils were investigated using a fermentor with a capacity of 10 L. The optimal conditions were as follows: glucose concentration 6% , substitute hydrolysate degree 0. 8 , air flow rate 0. 5 m /( m3 o min) , stirring speed 200 r/min, fermentation temperature (28 ± 1 )℃ and fermentation time 96 h. Under the optimal conditons, the biomass reached 19.43 g/L,the oil content reached 42. 37% ,and the oil yield was 8. 24 g/L. GC analysis showed that the main fatty acids were C16 and C18 fatty acids,and the biodiesel obtained by microbial oils reached the national standards.%研究玉米皮渣水解液高密度发酵生产微生物油脂的工艺条件,在10L发酵规模水平上探讨了玉米皮渣水解液取代度、通气量、搅拌速度对生产微生物油脂的影响.在糖浓度6％,玉米皮渣水解液取代度0.8,通气量0.5m3/(m3·min),搅拌速度200r/min,发酵温度(28±1)℃,发酵周期96h时,菌体生物量达19.43g/L,油脂含量为42.37％,油脂产量为8.24g/L.通过气相色谱分析微生物油脂的化学组成,主要成分为C16及C18脂肪酸,与植物油相似,同时转酯化制备的微生物柴油达到了国家标准.