A versatile method using immunoaffinity LC-MS/MS to quantify antigen protein in animal studies of monoclonal antibody therapeutics

摘要

A versatile immunoaffinity LC-MS/MS method has been developed for the bioanalysis of total antigen protein after dosing of mAb drug with LLOQ 3.13 ng/mL. This method requires only a commercial anti-human Fc region antibody that will capture the immune complex to measure total antigen protein.