ANALYSIS OF COCCIDIOSTATS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY

摘要

The use of sub-2(mu)m particle packed reversed phase column allow the separation of the eight coccidiostats in less than 7 min with high efficiency and low backpressure. The base peak of polyether ionophores in the single MS spectra is the sodium adduct, while chemical coccidiostats present the protonated (ESI +) or deprotonated (ESI -) molecule as base peak. The most abundant product ion for most of polyether ionophores in MS/MS corresponds to the loss of H_(2)O. For Maduramicin the main fragmentation is due to the simultaneous loss of two methoxy groups. Other characteristic products ions can also be observed except for Monensin.